Heart failure increases concentration of hydrogen peroxide in the tissues, which damages inner lining of the artery, also called the endothelium, and raising the possibility of blood clot creation and heart and brain strokes. Thus, concentration of hydrogen peroxide in body fluids such as plasma gains importance. The mortality rate by cardiovascular diseases in the country has been in rise which makes necessary an easy, rapid, and efficient method of determining the hydrogen peroxide concentration to prevent any damage to heat and brain.